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FOOD MICRO-ORGANISMS THEIR SIGNIFICANCE

Bacteria are not visible to the naked eye. They exist in and on the human body, in soil and in air, but only a few types can grow in food and cause food poisoning.

Under favourable conditions bacteria can multiply very rapidly simply by dividing in two every 20 to 30 minutes, so that one cell could produce up to 16 million cells within 8 hours.

With some bacteria, toxins are produced which are not destroyed by cooking – a food does not have to look, smell or taste ‘off’ to be potentially hazardous to health.

Total Viable Count (TVC)
TVC reflects the conditions in which the food was produced, stored or abused. With experience, this count can be used to predict the shelf life or keeping quality of the product. The spoilage of many foods may be imminent when the total viable count reaches 10- 100 million per g.

Coliform Counts
Coliform counts are generally used as an indicator of possible faecal contamination, and reflect the hygiene standards adopted in the food’s preparation. Improper handling and storage can allow the level to increase. Coliforms are also found on many types of plant material since the organisms are usually found at high levels in soil.

Escherichia coli (E. coli)
This is a faecal coliform which exists in the intestine of animals and man, and is an indicator of poor hygienic practice. Some strains eg E coli O157 can cause illness when present at levels as low as 10 per gram of food. These strains would not necessarily be included in traditional E coli tests: the very low infective dose means that cross contamination between foods is a particular hazard.

Staphylococcus aureus
This organism is commonly found on the skin, hair and mucous membranes, and very high concentrations are often found in wounds, sores or septic spots. They can readily be transferred to food by poor handling. Incubation time is short, and violent vomiting and diarrhoea can occur within 2 to 6 hours.

Salmonella
This organism accounts for over 50 per cent of all reported cases of food poisoning. Symptoms usually develop between 12 and 36 hours after ingestion, causing pain, diarrhoea, fever and vomiting which can last for 6 days or more.

Listeria
Although Listeria is widespread in the environment, it is only in recent years that the public have become aware of this organism. It has been involved in cases of food poisoning from the ingestion of affected milk, vegetables, meats, soft cheeses, ice cream and seafood. Listeria monocytogenes is the most significant species and can grow at refrigerator temperatures.

Yeasts and Moulds
Yeasts do not cause food poisoning, but some types are capable of causing food spoilage. Moulds can produce mycotoxins, some of which can affect man adversely. Mould spores can be carried by the wind, and hence can have easy entry to a factory.

"Faecal streptococci"
These organisms occurs in man and other animals. They do not multiply in water, but are more resistant to sanitation and heat than E. coli and therefore persist longer. The presence of faecal streptococci always acts as an indicator of faecal contamination.

Bacillus cereus
Bacillus cereus occurs in stools and is common in soil. Hence, foods such as rice, cereals and spices often contain this organism as can milk from cattle with mastitis. The organism is a spore former and can survive heat processing. Rapid growth of vegetative cells occurs at ambient temperatures. These cells produce toxins, both in the food and after ingestion.

Campylobacter
This organism is found in the digestive tract of animals and birds, and is the commonest cause of diarrhoea. Small numbers of these organisms (eg. 500) are sufficient to cause infection in man. The commonest sources of poisoning are poultry, eggs and dairy products. Contamination is usually caused by storing near raw meats or inadequate heat treatment. Symptoms usually occur between 2 and 7 days.

Clostridium perfringens
Clostridium perfringens is anaerobic, ie. it grows in the absence of oxygen. It is a very common organism, and is found in soil and the excreta of most mammals. The spores can survive in most conditions, and are found throughout the environment. They can survive cooking and the organism is therefore significant in cold meats. Its rate of growth is slow below 15 °C. It produces a toxin, causing symptoms of abdominal pain, diarrhoea and nausea between 8 and 24 hours after infection.

Results can be offered in a variety of formats including e-mail, first class post, fax and direct data transfer.
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Eclipse Food testing Laboratory offers a comprehensive range of UKAS accredited microbiological and chemical analyses, including:- 
Nutritional information labelling, vitamins and minerals, preservatives and other additives, contaminants & metals, toxins, pesticide and veterinary residues, pathogen testing, shelf-life studies, HACCP and food hygiene consultancies. Wide experience of serving the dairy industry. Monitoring of mineral and bottled waters, soft and alcoholic drinks.

 
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